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. 2020 Feb 7;28(1):41–49. doi: 10.3727/096504019X15615433287579

Figure 4.

Figure 4

Influence of LMNB2 on the biological behaviors of hepatocarcinoma cell lines. (A) Hep3b cells or SMMC7721 transfected with LMNB2 overexpression plasmid or siRNA, respectively. Western blot was used to detect protein level of LMNB2. The LMNB2 expression level was increased in Hep3B cells with overexpression LMNB2, and it was decreased in SMMC7721 cells transfected with siRNA (p < 0.05). (B) The images of colony formation assays (up) and quantification (bottom). The colony number was increased in Hep3B cells with overexpression LMNB2, and colony numbers was decreased in SMMC7721 cells transfected with siRNA (p < 0.05, respectively). (C) The results of migration detected by scratch assays. The results showed a significantly smaller or bigger width in the plasmid or siRNA of LMNB2 group compared with control in vitro (p < 0.05). (D) The results of invasion detected by Transwell assays. The results showed significantly more or less cells in the plasmid or siRNA of LMNB2 group compared with control in vitro (p < 0.05). *p < 0.05.