Table I.
Primer sequences used for reverse transcription-quantitative polymerase chain reaction.
| Gene name | Primer sequence (5' to 3') | Amplicon size (bp) |
|---|---|---|
| GAPDH | F: GCAAGTTCAACGGCACAGTCA | |
| R: ACGACATACTCAGCACCAGCAT | 127 | |
| HMGCR | F: CTTGACGCTCTGGTGGAATG | |
| R: GTTGGCAAGCACGGACATAC | 105 | |
| FDPS | F: AGCAGAATTTCATCCAGCACT | |
| R: GTCAGACCCCGATTGTACT | 148 | |
| RhoA | F: ATGTGGCAGATATTGAAGTGGA | |
| R: GTGTCTGGGTAGGAGAGAGG | 106 | |
| FGF-2 | F: ACCCACACGTCAAACTACAG | |
| R: GGCGTTCAAAGAAGAAACACTC | 150 | |
| TGF-β1 | F: CCTGAGTGGCTGTCTTTTGA | |
| R: CGTGGAGTACATTATCTTTGCTG | 124 | |
| VEGF | F: ACTGGACCCTGGCTTTACT | |
| R: ATTGGACGGCAATAGCTGC | 136 | |
| COL1A1 | F: GACGCATGGCCAAGAAGAC | |
| R: ACTTCTGCGTCTGGTGATAC | 234 | |
| HSP47 | F: GTCCATCAACGAGTGGGC | |
| R: CAGTGCGGCTTAAAGAACATG | 117 | |
| MVD | F: GTCAACATCGCGGTTATCAA | |
| R: CCTCTGTGAAGTCCTTGCTAA | 142 | |
| MVK | F: CCAAACGTCGGTATTAAGCA | |
| R: GCCTTCGTTGCCTACACA | 159 |
HSP47, heat shock protein 47; COL1A1, collagen type I α1; HMGCR, HMG-CoA reductase; FDPS, farnesyl pyrophosphate synthase; MVD, mevalonate diphosphate decarboxylase; MVK, mevalonate kinase; FGF-2, fibroblast growth factor-2; VEGF, vascular endothelial growth factor.