TABLE 2.
UPLC-Q-TOF/MS of two effective parts containing ethyl acetate and remaining parts of P. asiatica.
| Components | RT (min) | Selected ion | Measured mass (m/z) | Calc. mass (m/z) | Primary secondary fragment ion (MS/MS) and source |
|---|---|---|---|---|---|
| Plantamajoside | 5.5 | [M + Na]+ | 663.186 | 663.18956 | 663[M + Na]+, 501[M + Na-C6H10O5]+, 483[M + Na-C9H8O4]+ |
| Verbascoside | 5.7 | [M + Na]+ | 647.1923 | 647.19464 | 647[M + Na]+, 501[M + Na-C6H10O4]+ |
| Pratensein-7-O-glucoside | 6.2 | [M + H]+ | 463.1207 | 463.12349 | 463[M + H]+, 301[M + H-C6H10O5]+, 286[M + H-C6H10O5-CH3]+ |
| Hyperin | 4.7 | [M + H]+ | 465.10253 | 465.10275 | 465[M + H]+, 303[M + H-C7H14O4]+, 285[M + H-C7H14O4-H2O]+, 257[M + H-C7H14O4-H2O-CO]+, 169[M + H-C7H14O4-H2O-CO-C7H4]+ |
| Baicalin | 6.1 | [M + H]+ | 447.0905 | 447.09219 | 447[M + H]+, 271[M + H-C6H8O6]+, 153[M + H-C14H14O8]+ |
| Ursolic acid | 18.4 | [M + H]+ | 457.3643 | 457.36762 | 457[M + H]+, 411[M + H-CH2O2]+, 393[M + H-CH2O2-H2O]+, 203[M + H-CH2O2-H2O-C14H22]+ |
| Martynoside | 7 | [M + Na]+ | 675.2223 | 675.22594 | 675[M + Na]+, 529[M + Na-C6H10O4]+ |
| Apigenin | 8.3 | [M + H]+ | 271.0594 | 271.0601 | 271[M + H]+, 153[M + H-C8H6O]+, 145[M + H-C6H6O3]+, 119[M + H-C7H4O4]+ |
| Scutellarein | 5.3 | [M + H]+ | 287.0542 | 287.05501 | 287[M + H]+, 269[M + H-H2O]+, 169[M + H-H2O-C8H4]+, 123[M + H-H2O-C8H4-CH2O2]+, 119[M + H-H2O-C7H2O4]+ |
| Caffeic acid | 5.4 | [M + H]+ | 181.0483 | 181.04954 | 181[M + H]+, 163[M + H-H2O]+, 145[M + H-2H2O]+, 135[M + H-H2O-CO]+, 117[M + H-2H2O-CO]+, 107[M + H-H2O-2CO]+ |
| Ferulic acid | 7.4 | [M + H]+ | 195.0642 | 195.06519 | 195[M + H]+, 177[M + H-H2O]+, 149[M + H-H2O-CO]+, 145[M + H-H2O-CH4O]+, 134[M + H-H2O-C2H3O]+, 117[M + H-2H2O-C2H3O]+ |
| Luteolin | 7.4 | [M − H]− | 285.0366 | 285.03936 | 285[M − H]−, 217[M-H-C3O2]−, 199[M-H-C3O2-H2O]−, 175[M-H-C5H2O3]−, 151[M-H-C8H6O2]−, 133[M-H-C7H4O4]− |
| Calceorioside B | 1.3 | [M − H]− | 477.1567 | 477.13914 | 477[M − H]−, 315[M-H-C6H10O5]−, 161[M-H-C6H10O5-C11H6O]−, 133[M-H-C6H10O5-C11H6O-CO]− |
| Epimeredinoside A | 7 | [M − H]− | 651.2877 | 651.22835 | 651[M − H]−, 505[M-H-C6H10O4]−, 475[M-H-C6H10O4-CH2O]−, 193[M-H-C6H10O4-CH2O-C14H18O6]−, 175[M-H-C6H10O4-CH2O-C14H18O6-H2O]−, 160[M-H-C6H10O4-CH2O-C14H18O6-H2O-CH3]−, 134[M-H-C6H10O4-2CH2O-C14H18O6-H2O-CH3]− |