FIGURE 5.

SIRP-1 and SIRP-2 do not inhibit T cell proliferation. (A) Binding of 10 μg/ml Abs to cell-expressed SIRPγ was assayed using T-ALL cell line Jurkat and compared with benchmarks 18D5 and KWAR23. (B) Binding to naive or 72-h-activated human T cells was determined by cell-based binding assays and compared with SIRPγ-specific Ab clone LSB2.20. (C) A total of 10 μg/ml SIRP-1 or SIRP-2 did not inhibit T cell proliferation in an allogeneic T cell/dendritic cell assay over the course of 6–7 d. Each data point represents mean from a separate donor (shown in different shapes), constituting an average of six technical replicates within a minimum of two independent replicates. The asterisks (*) indicate statistical differences compared with the mIgG1 control group. Unless otherwise stated, all panels show mean ± SEM; a representative minimum n = 2 is shown. *p < 0.05.