Fig. 1.

PMA-stimulated THP-1 Differentiation. (A) Differentiation of monocytic cells into a macrophage-like phenotype by PMA stimulation. The number of adherent and suspended cells after 24 and 48 h of PMA treatment. (B) The effect of presence and absence of serum in the PMA-free culture medium during the resting stage on viable cell numbers. At first, cells were stimulated with PMA for 24 h (left side of Fig. 1B) and cells were maintained in serum-free or serum-containing medium for 24 h (right side of Fig. 1B).