Table I.
Genes | Primer sequences (5′→3′) |
---|---|
IL-1 | F: ATGGCAGAAGTACCTAAGCTCGC |
R: ACACAAATTGCATGGTGAAGTCAGTT | |
IL-6 | F: ACACACTGGTTCTGAGGGAC |
R: TACCACAAGGTTGGCAGGTG | |
TNF-α | F: ATGAGCACAGAAAGCATGATCCGC |
R: CCAAAGTAGACCTGCCCGGACTC | |
MMP1 | F: GCCACAAAGTTGATGCAGTT |
R: GCAGTTGAACCAGCTATTAG | |
MMP3 | F: ATGAAAATGAAGGGTCTTCCGG |
R: GCAGAAGCTCCATACCAGCA | |
MMP13 | F: ATGCATTCAGCTATCCTGGCCA |
R: AAGATTGCATTTCTCGGAGCCTG | |
ACTB | F: TGACGGGGTCACCCACACTGTGCCCATCTA |
R: CTAGAAGCATTTGCGGTGGACGATGGAGGG |
F, forward; R, reverse; MMP, matrix metalloproteinase; IL-, interleukin; ACTB, actin.