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. Author manuscript; available in PMC: 2021 Feb 2.
Published in final edited form as: J Vis Exp. 2020 Nov 3;(165):10.3791/61855. doi: 10.3791/61855

Figure 4. rAAV6 AAVS1 MND-GFP vector construct.

Figure 4.

Expression cassette contains a strong synthetic promoter (MND) sequence immediately followed by EGFP coding sequence and poly adenylation (Poly A) sequence. AAVS1 homology arms flank upstream of the MND promoter and downstream of the poly A sequences. EGFP will be expressed under the regulation of the MND promoter. Sequence lengths are indicated above each component of the construct.