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. Author manuscript; available in PMC: 2021 Feb 2.
Published in final edited form as: J Vis Exp. 2020 Nov 3;(165):10.3791/61855. doi: 10.3791/61855

Table 3.

Primers used

Description Sequence Use for
CD19 forward primers 5′-AAATTCAGGAAAGGGTTGGAAG-3′ Amplify the CD19 locus for Indel analysis
CD19 Reverse primer 5′-GCGGACCTCTTCTGTCCATG-3′ Amplify the CD19 locus for Indel analysis
Junction PCR forward primer 5′-GGACGAGCTGTACAAGTAACG-3′ Junction PCR
Junction PCR Reverse primer 5′-GAGACAGTGACCAACCATCC-3 Junction PCR