. 2021 Feb 2;5(1):e00303. doi: 10.1002/pld3.303

TABLE 2.

Pigment contents and photosynthetic parameters in the wild‐type and nfu1 mutants

	WT	nfu1−1	nfu1−2
ΔA _{830 nm} (PSI activity)	0.216 ± 0.009^A	0.196 ± 0.012^AB	0.171 ± 0.007^B
F _v/F _m (PSII activity)	0.781 ± 0.002^A	0.782 ± 0.002^A	0.778 ± 0.003^A
Total Chl (mg/g FW)	1.267 ± 0.019^A	1.255 ± 0.019^A	1.211 ± 0.027^A
Car (mg/g FW)	0.302 ± 0.005^A	0.295 ± 0.006^A	0.287 ± 0.006^A

Measurements of P700 photooxidation were performed on dark‐adapted, detached leaves, with the Dual‐PAM‐100 measuring system (Heinz Waltz GmbH). Far‐red light‐induced P700 photooxidation (ΔA _{830 nm}) is calculated as the absorbance change before and after a 39‐s illumination of saturating far‐red light (720 nm). Measurements of chlorophyll fluorescence parameters were performed on dark‐adapted plants, with the IMAGING‐PAM M‐Series chlorophyll fluorescence system (Heinz Waltz GmbH). Chlorophyll and carotenoid were extracted and determined as described by Wellburn (1994). Data are presented as means ± SE (n = 6 for P700 photooxidation, n = 8 for chlorophyll fluorescence parameters, and n = 5 for pigment contents). Values not connected by the same letter are significantly different (Student's t test, p < .05). Four‐week‐old plants were used for PSI activity, PSII activity, and pigment measurements in this table.

Abbreviations: Chl, chlorophyll; Car, carotenoid; FW, fresh weight.