. 2021 Feb 2;5(1):e00303. doi: 10.1002/pld3.303

TABLE 3.

Relative abundances of representative iron‐sulfur cluster‐containing proteins and other photosynthetic proteins in the wild‐type and nfu1 mutants

	WT	nfu1−1	nfu1−2
PsaA⁺⁺⁺⁺	1.00 ± 0.03^A	0.93 ± 0.02^AB	0.87 ± 0.02^B
PsaB⁺⁺⁺⁺	1.00 ± 0.05^A	0.87 ± 0.05^A	0.92 ± 0.03^A
PsaC⁺⁺⁺⁺	1.00 ± 0.10^A	0.74 ± 0.06^B	0.75 ± 0.04^B
D1	1.00 ± 0.05^A	1.12 ± 0.03^A	1.08 ± 0.04^A
CP43	1.00 ± 0.06^A	1.06 ± 0.04^A	1.10 ± 0.03^A
CP47	1.00 ± 0.04^A	1.04 ± 0.00^A	1.04 ± 0.02^A
PetB	1.00 ± 0.03^A	0.94 ± 0.02^A	0.91 ± 0.02^A
PetC^††	1.00 ± 0.10^A	1.08 ± 0.04^A	1.05 ± 0.03^A
cFD⁺⁺	1.00 ± 0.03^A	0.93 ± 0.02^A	0.98 ± 0.00^A
FD‐GOGAT⁺⁺⁺	1.00 ± 0.02^A	0.90 ± 0.03^B	0.95 ± 0.02^AB

Proteins were immunodetected as in Figure S1. The values (mean ± SE, n = 4) are given as ratios to the protein levels in the wild type (WT). Leaf total proteins loaded on an equal total protein basis were used to determine the abundances of cFD and FD‐GOGAT. Because the nfu1 mutants did not display changes in chlorophyll contents, thylakoid membrane proteins loaded on an equal chlorophyll basis were used to determine the levels of other proteins in this table. Symbols ++, ††, +++, and ++++ indicate that the protein binds classic 2Fe‐2S, Rieske‐type 2Fe‐2S, 3Fe‐4S, and 4Fe‐4S, respectively. Values not connected by the same letter are significantly different (Student's t test, p < .05). Four‐week‐old plants were used for SDS‐PAGE and immunoblot analysis in this table.