FIGURE 5:

VPS13A^mCherry localization to lipid droplets is altered by GFP-XK expression. HEK293T cells were transfected with VPS13A^mCherry (pVPS13A^mCherry) without (left panels) or with (right panels) mTagBFP-XK (pJS143-E6) and stained with the lipid droplet dye BODIPY 493/503. Yellow arrows highlight examples of VPS13A^mCherry foci and pink arrows highlight examples of lipid droplets stained with BODIPY Green. Numbers below indicate the percentage of VPS13A^mCherry foci overlapped with the lipid droplet marker ± range. Data are the average of two independent experiments. In the absence of GFP-XK 255 foci were scored, while 96 foci were scored for cells containing GFP-XK. Scale bars = 10 μm. For illustration purposes, the cells shown have a greater than average number of VPS13A^mCherry foci (Figure 4).