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. 2020 Oct 15;31(22):2425–2436. doi: 10.1091/mbc.E19-08-0439-T

FIGURE 6:

FIGURE 6:

VPS13A^mCherry and GFP-XK colocalize at the ER and mitochondria. HEK293T cells cotransfected with VPS13A^mCherry and GFP-XK or mTagBFP-XK were either stained with the lipid droplet marker BODIPY 493/503 or cotransfected with an additional plasmid expressing an ER marker (pEFIRES-mTagBFP-KDEL) or a mitochondrial marker (mito-BFP). (A) Representative cell showing the localization of a VPS13A^mCherry/mTagBFP-XK focus (yellow arrow) relative to a lipid droplet. (B) Representative cell showing the localization of a VPS13A^mCherry/GFP-XK focus (yellow arrow) relative to the ER. (C) Representative cell showing the localization of a VPS13A^mCherry/GFP-XK focus (yellow arrow) relative to the mitochondrial marker. Scale bars = 10 μm.