FIGURE 5:

A functional NM2C motor domain is required for microvillar length regulation. SIM MaxIP images of representative Ls174T-W4 cell overexpressing (A) EGFP as a negative control, (B) WT Halo-NM2C, (C) WT EGFP-NM2A, (D) Halo-NM2C-E479A, (E) Halo-NM2C-N252A, or (F) Halo-NM2C-R874A. All Halo-NM2 constructs are labeled with JF585 (green), and cells are also costained with phalloidin to visualize F-actin (magenta). All scale bars = 5 μm. (G) Microvillar length quantification for each overexpression condition; measurements were made from 10 cells per construct, 8–10 microvilli measured per cell. Each data point is a single microvillus. Testing was performed using an ordinary one-way ANOVA with Dunnett’s multiple comparisons test; ****, p < 0.0001 vs. WT NM2C.