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Related to Figure 4. Microvillar core actin bundles are rapidly turned over. Spinning disk confocal imaging of a FRAP control experiment on induced Ls174T-W4 cell expressing mNeonGreen β-actin. ROI is bleached using a 405-laser line at 30% power with 100 μs dwell time. 5 frames acquired pre-bleach at 60 second intervals;22 frames acquired post-bleach at 30 second intervals for 10 minutes. Playback is 3 frames per second.
