Movie S6.

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Related to Figure 4. Inhibition of NM2 limits actin turnover. Spinning disk confocal imaging of a FRAP experiment on induced Ls174T-W4 cell expressing mNeonGreen-β-actin, treated with blebbistatin for 15 minutes prior to imaging. ROI is bleached using a 405-laser line at 30% power with 100 μs dwell time. 5 frames acquired pre-bleach at 60 second intervals; 22 frames acquired post-bleach at 30 second intervals for 10 minutes. Playback is 3 frames per second.