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. 2020 Nov 1;31(23):2597–2629. doi: 10.1091/mbc.E18-01-0013

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FIGURE 5: — ER stress does not elicit IRS1 S307/S312 phosphorylation. (A, B) Serum-starved 3T3-F442A cells, (C, D) C₂C₁₂ myotubes, and (E, F) Hep G2 cells were treated with 1 μM thapsigargin or 5 μg/ml anisomycin (ANI) for the indicated times. (A, C, E) Cell lysates were analyzed by Western blotting. (B, D, F) Quantification of the Western blots shown in panels A, C, and E. Bars represent SEs (n = 3). p values for comparison of every sample to every other sample were calculated by ordinary one-way ANOVA with Tukey’s multiple comparisons test. Anisomycin-treated samples are positive controls in the phospho-S307/S312-IRS1 Western blots.