FIGURE 4:

Inhibition of PIKK-family kinases does not prevent phosphorylation of the FUS prion-like domain following osmotic or oxidative stress. (A) Phosphorylation status of FUS from H4 cells treated with or without torin 2 under varying stress conditions were analyzed by Western blot. (B) Quantification of band fluorescence normalized to total FUS; error bars represent 95% CI (n = 3). (C) Phosphorylation of FUS in H4 cells treated with or without Torin 2 under varying stress conditions. Fixed cells imaged using confocal microscopy. Cells were probed with FUS and phospho-FUS(pS30) antibodies. (D) Quantification of nuclear and cytoplasmic phospho-FUS(pS30); fluorescence error bars represent 95% CI (n = 3).