Fig. 6.
SKO treatment significantly increased the auto-secretion of TNF-α in MDA-MB-231 cells to induce cell apoptosis and necrosis. a Immunoprecipitation assay of the interactions between RIP1 and cIAP2. b The expression of phosphorylation IKKα/β in cells treatment with or without of SKO at the indicated concentrations. c Semi-quantitation the expression of phosphorylation IKKα/β in cells treatment with or without of SKO at the indicated concentrations. d The gene expression of TNF-α in cells treated with or without of SKO at the indicated concentrations. e The concentration of TNF-α in culture medium. Data was represented as means ± SEM. **p < 0.01 vs. untreated control group