Fig. 7.

LINC00460 governs CRC growth and metastasis by stabilizing HMGA1 mRNA in vivo. a, b, c Effect of LINC00460 KD and LINC00460 KD with overexpression HMGA1 in HCT116 cells on the xenograft model was assessed by evaluating tumor volume and tumor weight. 5 × 10⁶ HCT116 LINC00460 Con/KD cells and LINC00460 KD with overexpression HMGA1 and Matrigel (Corning; 1:1 ratio) were subcutaneously injected into the abdominal flanks of each mice, respectively (**p < 0.01). d The tumor sections were performed immunochemistry staining by antibody against HMGA1, representative images were shown. e Western blot analysis of HMGA1 expression in tumor xenografts formed. GAPDH was used as a loading control. f, g Representative bioluminescence images and statistical analysis of lung metastases in mice via tail vein injection of indicated cells (**p < 0.01, ***p < 0.001). h HCT116 LINC00460 Con/KD cells and LINC00460 KD with overexpression HMGA1 cells were injected via the lateral tail veins. Representative lung images (up) at week 6, corresponding hematoxylin eosin stained lung sections are shown (low). Arrows, lung metastasis foci. i Statistical analysis of lung metastasis foci (**p < 0.01, ***p < 0.001). j Kaplan-Meier survival curves depicting overall survival of the mice with injection of HCT116 LINC00460 Con/KD cells and LINC00460 KD with overexpression HMGA1 (**p < 0.01, ***p < 0.001)