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. 2020 Oct 1;31(21):2315–2330. doi: 10.1091/mbc.E20-04-0252

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© 2020 Bancroft, Holder, Geraghty, et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License.

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FIGURE 1: — PP1 counteracts CDK1 regulation of APC/C during mitotic exit. (A, B) HeLa cells were arrested in mitosis and incubated for 25 min with either (A) DMSO (Control) or (B) 5 µM tautomycetin PP1 inhibitor (PP1-i). CDK1 was then inhibited (CDK-i) using 5 µM flavopiridol, and samples of the culture were taken at the times indicated and Western blotted for the indicated markers. (C) PP1α-pT320 and (D) CCNB1 levels were measured as a function of time and plotted in the line graphs for the representative experiments shown in A and B.