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. 2020 Oct 1;31(21):2315–2330. doi: 10.1091/mbc.E20-04-0252

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© 2020 Bancroft, Holder, Geraghty, et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License.

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FIGURE 3: — PP1 is needed for rapid destruction of cyclin B at the metaphase–anaphase transition. (A, B) Comparison of the time spent (A) in mitosis or (B) to enter anaphase following completion of a metaphase plate in siControl, siPP1α/γ depletion, or siPP1β depletion for CRISPR-tagged CCNB1-GFP HeLa cells. Scatter plots of mean ± SD are shown for siControl (n = 17), siPP1α/γ (n = 30), and siPP1β (n = 20). ** denotes p < 0.001. (C) Live cell imaging of CCNB1-GFP with times shown in minutes. Percentage of CCNB1-GFP fluorescence following NEBD in (D) siControl, (E) siPP1α/γ, and (F) siPP1β cells. Brightfield images (BF) show the cell outline. CCNB1 images are maximum-intensity projections; quantification was carried out on sum intensity projections, and the single-cell traces were plotted as a function of time from NEBD in the graphs.