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. 2020 Oct 1;31(21):2315–2330. doi: 10.1091/mbc.E20-04-0252

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© 2020 Bancroft, Holder, Geraghty, et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License.

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FIGURE 6: — In the absence of PP1α/γ, cyclin B destruction is delayed when the spindle checkpoint is inactivated by MPS1 inhibition. (A) HeLa CCNB1-mCherry GFP-MAD2 cells treated with control or siPP1α/γ were imaged progressing through mitosis and treated with MPS1 inhibitor AZ3146 (MPS1-i) when they approached metaphase. Loss of GFP-MAD2 and kinetics of CCNB1-mCherry degradation were monitored. (B) CCNB1-mCherry levels in single cells are plotted for siControl and siPP1α/γ cells undergoing mitotic exit. The time of MPS1-i addition (t = 0) is marked with a dashed line. (C) The percentage of cyclin B1-mCherry remaining at 30 min after MPS1-i addition in siControl (n = 19) and siPP1α/γ (n = 24) cells was plotted as mean ± SD in the line graph. A p value was calculated with an unpaired t test with Welch’s correction. **** denotes p < 0.0001.