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. 2021 Feb 1;9:37. doi: 10.1186/s40168-020-00990-y

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Boxplot of different features across non-viral and viral groups. “Nonviral” includes bacteria and archaea, fungi and protozoa, and plasmids. A subset of 100 random genome fragments were used for each group. “% of viral gene” is calculated as the percent of genes annotated as viral (best hit to viral HMMs) of all genes; “% of bacterial gene” is calculated as the percent of genes annotated as bacterial (best hit to bacterial HMMs) of all genes; “Strand switch frequency” is the percent of genes located on a different strand from the upstream gene (scanning from 5′ to 3′ in the + strand); “Gene density” is the average number of genes in every 1000 bp sequence (total number of genes divided by contig length and then multiplied by 1000); “Average GC content of genes” is the mean of GC content of all genes in a contig; “TATATA_3-6 motif frequency” is the percent of ribosomal binding sites (RBS) with “TATATA_3-6” motif