Fig. 5.

Surface expression of FLT3 determines therapy response. a Gating strategy of mass cytometry analysis for FLT3 high and FLT3 low expressing MOLM-13 cells after 18 h in vitro culture. b Graph showing quantified change in expression illustrated as log₂ median intensity compared to control in FLT3 high and FLT3 low expressing MOLM-13 cells cultured with indicated treatments; PKC412 10 nM, Dauno 0.05 µM, PKC412 10 nM plus Dauno 0.05 µM, AraC 5 µM, PKC412 10 nM plus AraC 5 µM. ±SD (n = 4), *, P ≤ 0.05 **, P ≤ 0.01, as measured by Student’s t test. c Experimental set up and heatmap of in vitro intracellular change in phosphoprotein expression intensity during 18 h treatment (as indicated in the figure) in FLT3 high vs. FLT3 low expressing FLT3-ITD positive AML patient cells. Calculated transformed ratio of medians by first row using channel values (green correspond to high expression and red low)