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. 2021 Feb 1;9:19. doi: 10.1186/s40478-021-01119-4

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Reduction of Nlgn1 band in parietal cortex of AD brain. a Detection of Nlgn1 with antibodies targeting different epitopes by immunoblot. TBS soluble fractions of 4 Ctrl and 4 AD brain samples, 20 µg protein/lane. Samples 3–8–17–2–10–11-14 are from superior parietal cortex, while sample 7 is from inferior parietal cortex. a The blue-boxed areas indicate the common Nlgn1 immunoreactive band at around 80 kDa. Recombinant Nlgn1 protein, corresponding to the extracellular part fused to a C-terminal 6-His tag, is shown in the first lane. b Significant reduction of Nlgn1 band intensity, detected by a-Nlgn1 antibody from Synaptic System. c Significant reduction of Nlgn1 band intensity, detected by a-Nlgn1 (F-7) antibody. The bars presented in the scatterplots show the median and the interquartile range. Significance: p ≤ 0.05. d–e Western blots showing the specificity of F-7 and SySy a-Nlgn1 antibodies toward Nlgn1 recombinant protein. Ab = antibody