Fig. 6.

SDS-page gel (Coomassie-stained) for protein visualization and band excision for protein identification using mass spectrometry. Highlighted in the figure are the excised gel segments for the recombinant protein (1), TBS brain extract (2 to 7), and CSF sample (8 to 14). To avoid cross-contamination and protein diffusion between lanes, samples were loaded with two empty lanes in between them. Control gel segments were also cut (4, 11, 15, 16, negative controls), to further check for protein contamination between lanes. The excised gel segments were digested and protein extracted for MS analysis