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. 2020 Sep 30;8:100047. doi: 10.1016/j.mbplus.2020.100047

Fig. 6.

Fig. 6

Multicellular aggregate circularity assay. (A) Schematic of multicellular aggregates containing 344SQ cells alone or in combination with CAFs seeded on defined MC cell-derived collagen matrices. (B) Fluorescent confocal micrographs of multicellular aggregates taken three days after seeding on matrices generated by MC cells transfected with control shRNA (shCTL), LH2 shRNA (shLH2), or LH3 shRNA (shLH3). Scale bar size, 100 μm. (C) Representative images of image processing for circularity quantification. Fluorescent image of 344SQ cells in multicellular aggregate (left) is converted into eight-bit image (center) and thresholded to generate a mask (right) suitable for shape-descriptor analysis. (D) Dot plot of circularity values of each multicellular aggregate (dot) containing 344SQ cells alone or in combination with CAFs seeded on matrices generated by shCTL-, shLH2-, shLH3-transfected MC cells. Two wells/condition, n = 10 aggregates/condition.