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. 2019 Jul 25;4:100011. doi: 10.1016/j.mbplus.2019.100011

Fig. 6.

Fig. 6

Effect of EMILIN1 or Fibrillin1 depletion from the extracellular matrix on renal epithelial cell growth and migration. (A) ki67/DAPI ratio as measure for cell proliferation at 24, 48 or 72 h post seeding in HRPTECs cultured on siSHAM, siEMILIN1 and siFBN1 ECM. Shown is mean ± SEM. N ≥ 26 fluorescent images derived from N = 3 samples. (B) Viability measurements using the PrestoBlue assay after 24, 48 or 72 h post-seeding in HRPTECs on siSHAM, siEMILIN1 and siFBN1 ECM. Shown is mean ± SEM. N = 6 assays. (C) DNA abundance measurements using the PicoGreen assay after 24, 48 or 72 h post-seeding in HRPTECs on siSHAM, siEMILIN1 and siFBN1 ECM. Shown is mean ± SEM. N = 5 assays. Quantified live-cell migration assay results showing velocity and covered and Euclidean distance of HK2 cells on either siEMILIN1 (D-F) and siFBN1 (G-I) ECM compared to cells on siSHAM ECM. The migration of HK2 cells on the ECM layer was tracked overnight with a confocal microscope. Shown is mean ± SEM; *P < 0.05, **P < 0.01. N = 60 individual cells tracked derived from N = 4 assays. Migration plots showing multiple tracks of individual HK2 cells on siSHAM (J), siEMILIN1 (K) and siFBN1 ECM (L).