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. 2019 Jul 6;3:100009. doi: 10.1016/j.mbplus.2019.100009

Fig. 1.

Fig. 1

Loss of CCN1 in fibroblasts in mice leads to decreased skin thickness. A) Genotyping showing loss of CCN1 expression in fibroblasts two weeks post-tamoxifen injections. Fibroblasts extracted from skin of mice show loss of CCN1 as revealed by analysis of B) mRNA (P = 0.0122; Graph shows mean fold change +/− SD; Student t-test; n = 6); and C) protein (representative image, n = 3) expression. D) In mice, 4 weeks post-injection of tamoxifen (i.e., at 8 weeks of age), loss of CCN1 results in translucent skin. (Representative image; n = 4). E) Dermal layer of skin is significantly thinner (p = 0.0005) in CCN1−/− mice as revealed by H&E staining. (Graph shows mean dermal thickness +/− SD; Student's t-test; n = 4). F) There is an increase in Young's modulus (stiffness) that is increased (p = 0.0354, n = 4) in the absence of CCN1 but is unlikely to be of biological significance and G) more nuclei present (p = 0.0013) per unit area of skin in CCN1−/−, compared to CCN1f/f, mice as shown by DAPI staining of. (Representative images shown; Graph shows mean cells per 120,000 μm2 +/− SD; Student's t-test; n = 5). H) No alterations were observed in the proliferation marker PCNA. (Representative images shown; Graph represents mean percentage of cells PCNA positive +/− SD; Student's t-test; n = 5). I) Tunel assay showed no alteration in apoptosis. (Representative images shown; n = 6).