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. 2019 Sep 4;4:100014. doi: 10.1016/j.mbplus.2019.100014

Fig. 2.

Fig. 2

SMC transcriptional changes after 30 days of ASC-SF stimulation of SMCs. (A) Gene expression related to SMC phenotype. RT-qPCR revealed an increase in myosin heavy chain (MYH11) after ASC-SF stimulation, while both α smooth muscle actin (ACTA2) and calponin (CAL) were unchanged. n = 3. (B) Gene expression related to elastic fiber formation. RT-qPCR revealed increases in expression of tropoelastin induced by both ASC-SF and NCM, as well as ASC-SF induced increases in microfibril protein fibrillin-1, organizational matricellular protein fibulin-5, and cross-linking proteins lysyl oxidase (LOX) and lysyl oxidase-like 1 (LOXL-1). Fibulin-4 expression was unchanged with both ASC-SF stimulation and NCM when compared to NT, and LTBP-4 expression was significantly reduced with both ASC-SF stimulation and NCM.