Fig. 5.

Shift in strand mechanical properties after ASC-SF stimulation. (A) Photo of fibrin removed from top half of nylon tabs of TissueTrain plates, resulting in an exposed tab that can be dried and clamped by sandpaper-aided pneumatic clamps. (B) Image of tensile testing configuration (Instron #5543A, with sandpaper-lined pneumatic clamps) used to test 30-day NT and ASC-SF treated strands, with ruler for scale. (C) For each sample group, the range of maximum and minimum stress values was plotted as a pair of thick lines, with the average values plotted as small circles. “Low” and “High” stretch regions are indicated, for each sample this was the first and final 1/3 of data points, respectively. (D) Comparison of low and high stretch elastic modulus for both 30-day NT and ASC-SF stimulated strands, alongside cell-free fibrin gel constructs.