Figure 1: Overview of nucleic acid extraction from saliva specimens —

a, Overview of Innovative Genomics Institute’s (IGI’s) specimen processing pipeline for both swab and saliva samples. OP = oropharyngeal. OP-MT = oropharyngeal-mid turbinate. b, Cultured SARS-CoV-2 (1.58×10⁶ TCID50/ml) was mixed 1:1 with OMNIgene solution present in OM-505 collection tubes to test incubation conditions that inactivate viral replication. Samples were either held at room temperature (RT) or incubated at 65°C for the indicated length of time before being applied to Vero-E6 cells. Cytopathic effect (CPE) was quantified at 3 and 7 days post treatment (dpt). c, 3:2 dilution of saliva samples with DNA/RNA Shield improves detection of spiked-in SARS-CoV-2 RNA or MS2 in four saliva donors.