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[Preprint]. 2021 Feb 1:2021.01.29.21250790. [Version 1] doi: 10.1101/2021.01.29.21250790

Figure 5.

Figure 5.

Viral RT stability in saliva by RT-ddPCR assays. (A) Virions were added to saliva samples at 1000 virion genome copies/μl and stored at room temperature. Samples were heated with 1/5 volumes of 50% Chelex prepared in H2O or TED99 on the time points indicated and assayed. The RT-ddPCR reactions were carried out in one well for N1 and cRPP30 and another well for N2 and RPP30. (B) Virions of 1000 virion genome copies/μl were prepared in saliva as in (A), heated with Chelex on day 0 or day 3, and assayed on the days indicated. cRPP30 data points were not plotted because of the low level detected. Copies/μl refers to concentration in the samples used for RT-ddPCR. The error bars represent Poisson 95% confidence intervals.