Fig. 7.

Broadband UVB irradiation of HDF-derived microfibril isolations may also lead to significant changes in the proteolytic susceptibility of co-purifying microfibril-associated receptors integrins αv and β1. LC-MS/MS-identified peptide sequences (PSMs: peptide prophet FDR ≤ 5%) in UVB-irradiated, SSR irradiated and control HDF-derived microfibril isolations were counted for each respective protein domain and normalised based on total spectrum count (N = 3; graphs = mean PSM count per protein domain and SD; statistical comparisons were made using Bonferroni-corrected multiple comparisons tests). Compared to control, broadband UVB-irradiation yielded significantly more PSMs at domain FG-GAP 6 (p = 0.0364) of integrin αv (Ai) and at the vWFA domain of integrin β1 (p = 0.023; Aii). SSR-irradiation however, failed to induce any significant changes in the PSM counts within domains of integrin αv (Bi) or integrin β1 (Bii).