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. 2019 Nov 20;4:100017. doi: 10.1016/j.mbplus.2019.100017

Fig. 2.

Fig. 2

mLH 7:2pAb recognizes murine but not human collagen VII. A, Human and murine keratinocytes and human and murine skin cryosections all wild-type stained with mLH 7:2pAb and hLH 7:2pAb at 1:20,000 dilutions. Note that at this dilution hLH 7:2pAb recognizes human but not murine collagen VII and mLH 7:2pAb recognizes murine but not human collagen VII. Nuclei counterstained with DAPI, scale bar = 50 μm. B, mLH 7:2pAb and hLH 7:2pAb show species selectivity in western blotting. Western blots of protein lysates from normal human keratinocytes or keratinocytes from donors with complete collagen VII deficient recessive DEB (RDEB), or protein lysates from wild-type or collagen VII hypomorphic mouse skin. The blots were probed with hLH 7:2pAb and mLH 7:2pAb as indicated at a dilution of 1:5000. β-tubulin was used as loading control.