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. 2020 Jan 17;6-7:100022. doi: 10.1016/j.mbplus.2020.100022

Fig. 1.

Fig. 1

IGF-IR depletion in metastatic T24T cells only partially inhibits motility and anchorage-independent growth. (A) IGF-IR depletion in T24T cells was obtained by stable transfection of the pRS/shIGF-IR plasmid or control and subsequent selection in puromycin-containing media. (B) Migration of T24T cells was assessed in Boyden chambers as described [49,80,81].*p < 0.05. (C) Anchorage-independent growth was measured by soft-agar assays. Colonies ≥150 μM were counted [82]. Experiments are the average of three independent experiments ±SD. Bar~200 μm. *p < 0.05. (D) Representative colonies generated from shScr or shIGF-IR#4 transfected T24T cells. (E) IGF-IR and IR expression levels in various urothelial carcinoma-derived cells were assessed by immunoblot with anti-IGF-IR and anti-IR polyclonal antibodies. β-Actin served as the loading control.