Table 2.

Determination of the optimal coating amount of PCV2-Cap protein and the optimal dilution of PCV2-Nb15-HRP fusion protein

Dilution of PCV2-Nb15-HRP	The OD450nm value of different amounts of PCV2-Cap protein (µg/mL)
	0.5	1	2	4	8
1:100	2.109 ± 0.062	2.283 ± 0.004	2.536 ± 0.026	2.688 ± 0.049	2.874 ± 0.020
1:200	1.620 ± 0.088	1.962 ± 0.010	2.014 ± 0.034	2.316 ± 0.173	2.804 ± 0.015
1:400	1.170 ± 0.011	1.304 ± 0.018	1.435 ± 0.037	1.684 ± 0.019	2.490 ± 0.014
1:800	0.451 ± 0.074	1.098 ± 0.001	1.193 ± 0.024	1.35 ± 0.038	1.727 ± 0.029
1:1600	0.264 ± 0.038	0.477 ± 0.009	0.522 ± 0.003	0.686 ± 0.005	1.165 ± 0.053
1:3200	0.180 ± 0.020	0.209 ± 0.008	0.215 ± 0.004	0.396 ± 0.011	0.634 ± 0.011

The italics represent the selected concentration of antigen and the dilution of nanobody