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. 2020 Oct 2;6(40):eabb3298. doi: 10.1126/sciadv.abb3298

Fig. 5. Triencapsulation of ovarian cancer cells, HUVECs, and hMSCs in PA/KN hydrogels and Matrigel (ovarian cancer/HUVEC/hMSC ratio of 560:6000:600).

Fig. 5

(A) Quantification of F-actin network coverage as percentage area coverage for different PA/KN hydrogel combinations. (B) Metabolic activity of tricultures over time in PA-VH/PA-RGDS/KN/FN hydrogels and Matrigel (values normalized to day 1). (C) Live (green)/Dead (red) images of tricultures in PA-VH/PA-RGDS/KN hydrogels and Matrigel on days 1, 7, and 14. (D) IF staining of F-actin network (phalloidin/red) and nuclei (DAPI/blue) of tricultures grown in PA-VH/PA-RGDS/KN/FN hydrogels and Matrigel on days 1, 7, and 14. (E) IF staining for CD31 (green), F-actin network (phalloidin/red), and nuclei (DAPI/blue) in Matrigel on day 4. (F) IF staining for CD31 (green), F-actin network (phalloidin/red), and nuclei (DAPI/blue) in PA-VH/PA-RGDS/KN hydrogel on day 7 with a 3D volume reconstruction (right). (G) Still video images of cell proliferation and spreading in PA-VH/PA-RGDS/KN hydrogels from days 2 to 7 (HUVECs labeled with a green cell tracker, F-actin network with red phalloidin, and nuclei with blue DAPI).