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. 2021 Feb 1;220(3):e202004196. doi: 10.1083/jcb.202004196

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© 2021 Rüthnick et al.

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Figure S1. — Y2H interaction study of N-Sfi1. (A) Uncut Y2H assay with all analyzed SPB components. Parts are shown in Fig. 1 B. The full-length Spc42 Y2H construct did not interact with Sfi1_1–187 and Sfi1_1–296, because this construct is nonfunctional, since it does not show a positive interaction with Spc29 and Cnm67 (Elliott et al., 1999). (B) Immunoblot to confirm the expression of all Sfi1 constructs analyzed in Fig. 1 C and Fig. 3 B. Tub4 is the loading control.