Figure S1.

Flowchart of patient enrollment, data acquisition, and analytic pipeline. In TRACK-SCI, as soon as a confirmed SCI patient is admitted and consents to participate in the study, our team collects clinical data during all stages of the hospital stay and at 3, 6, and 12 mo after injury (in total >22,000 data points for each patient). Blood is also collected as early as possible after hospital admission (day 0) and at days 1, 2, 3, and 5 as well as at 6 and 12 mo after injury. After the blood draw, WBCs are isolated, and RNA is extracted for RNA-seq. The RNA-seq data from SCI patients along with RNA-seq data from HCs and TCs are analyzed using both supervised and unsupervised methods with the goal of creating a predictive model for injury severity. ED, emergency department; ICU, intensive care unit; Moberg, data collected from the Moberg ICU device; MRI, magnetic resonance imaging; NQoL, quality of life in neurological diseases; OR, operating room; ZSFG, Zuckerberg San Francisco General Hospital and Trauma Center.