Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Feb 1;153(3):e202012726. doi: 10.1085/jgp.202012726

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021 Rahmanseresht et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 3. — Relative positions of actin and MyBP-C in sarcomeres from active muscle preparations determined by combining STORM with particle averaging. (A) Examples of partial hexagons cropped from active muscle sarcomeres in the actin STORM images. (B) Average reconstructed actin fluorescence image generated from 120 actin ROIs originating from different sarcomeres. (C) 3-D plot of the average actin image shown in B, with the z axis (intensity) representing localization certainty. The distance between two neighboring actin filaments was 29 ± 1 nm. (D) ROIs of the MyBP-C reconstructed images cropped from areas identical to those shown for actin in A. (E) Average reconstructed MyBP-C image, as in B for the actin. (F) 3-D plot of the average MyBP-C image shown in E. (G) Merged images shown in B and E. Actin (green) and MyBP-C (magenta). (H) Merged 3-D images shown in C and F. Actin (green) and MyBP-C (magenta). Scale bars, 25 nm. a.u., arbitrary unit.