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. 2021 Feb 1;153(3):e202012726. doi: 10.1085/jgp.202012726

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© 2021 Rahmanseresht et al.

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Figure S2. — Longitudinal and cross-sectional views of mouse cardiac papillary muscle. (A and B) Negative-stain EM images of 100-nm-thick cryo-sections, longitudinal (A) and transverse (B), of mouse papillary muscle from active and relaxed muscle preparations, respectively, demonstrating the actin and myosin filament lattice within the myofibrils. Scale bars, 1 µm (A) and 500 nm (B). (C and D) Reconstructed images of Alexa Fluor 488-phalloidin (actin) filaments (C) and Alexa Fluor 647 anti-myc (MyBP-C’s C0 domain; D) within 400-nm-thick transverse cryo-sections of mouse papillary muscle imaged by STORM. Scale bars, 5 µm.