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. 2021 Feb 1;153(3):e202012726. doi: 10.1085/jgp.202012726

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© 2021 Rahmanseresht et al.

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Figure S6. — Relative positions of actin and MyBP-C in sarcomeres from relaxed and active muscle preparations determined by combining STORM with particle averaging. (A and B) Average reconstructed actin image (A) and 3-D plot (B) generated from 134 actin hexagons originating from different relaxed muscle sarcomeres in the hearts treated with blebbistatin before fixation. (C and D) Average reconstructed MyBP-C image (C) and 3-D plot (D) from areas of the MyBP-C images corresponding to those shown for actin in A. The z axis is intensity, representing localization uncertainty. (E) Merged images shown in A and C. Actin (green) and MyBP-C (magenta). (F) Merged 3-D images shown in C and D. Actin (green) and MyBP-C (magenta). (G–L) Similar data to that shown in A–F but from active muscle sarcomeres. 120 individual actin reconstructions were used to generate the average images. The interactin distances and uncertainties were similar between the active and relaxed sarcomeres. a.u., arbitrary unit.