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. Author manuscript; available in PMC: 2022 Jan 1.
Published in final edited form as: Nat Rev Urol. 2020 Nov 19;18(1):46–66. doi: 10.1038/s41585-020-00390-9

Fig. 3 ∣. Capacitation-associated protein tyrosine phosphorylation.

Fig. 3 ∣

a ∣ Tyrosine phosphorylation (P-Tyr) exhibits subflagellar localization. A cross-sectional view of the principal piece (left) shows CATSPER (red), fibrous sheath (blue), outer dense fibres (brown), and 9+2 axoneme (line structures in the centre). A two-colour 3D stochastic optical reconstruction microscopy (STORM) cross-sectional image of a capacitated wild-type spermatozoon (right) shows CATSPER1 (red) and P-Tyr (green). Note that P-Tyr is localized in the centre of the cross-section, corresponding to the axoneme. b ∣ P-Tyr identified by quantitative whole-sperm proteome analysis from capacitated wild-type and Catsper1-null mice. Each protein is represented as a dot in a volcano plot of statistical significance (y-axis) against the average protein fold-change (x-axis) of Catsper1-null compared with wild-type spermatozoa. Four axonemal proteins (more than twofold change and P < 0.05) are marked in colour.