Table 2.
Methods for the derivation of organoids from healthy tissues of the human FRT and the placenta.
| Type | Tissue source | Digestion method | Substrate | Reference |
|---|---|---|---|---|
| Ovarian organoids | Biopsies from women with/without BRCA1/BRCA2 mutations undergoing prophylactic bilateral salpingo-oophorectomy | Mechanical (mincing) and enzymatic (Collagenase XI with ROCK inhibitor Y-27632) | 10 mg/ml Cultrex® Basement Membrane Matrix, Type 2 | [69] |
| Fallopian tube organoids | Anatomically normal biopsies from women with benign gynecological disease | Enzymatic (Collagenase I) and mechanical (scraping) | 100% Matrigel® | [71] |
| Endometrial organoids | Decidua in 8–12 weeks gestation from normal pregnancy | Mechanical (mincing) and enymatic (Dispase II and Collagenase V) | 100% Matrigel® | [78] |
| Endometrium in secretory, proliferative, and postmenopausal phase from non-hormonally treated women | Mechanical (mincing) and enzymatic (Dispase II and Collagenase V) | 100% Matrigel® | [78] | |
| Endometrium in proliferative phase from non-hormonally treated women | Mechanical (mincing) and enzymatic (Collagenase IV and TrypLE with Y-27632) | 70% Matrigel® | [77] | |
| Decidua in 8–11 weeks gestation from normal pregnancy | Mechanical (mincing) and enzymatic (Collagenase I with DNase I) | 60% Matrigel® | [79] | |
| Cervical organoids | Anatomically normal biopsies | Mechanical (mincing) and enzymatic (Collagenase II and TrypLE Express) | 100% Matrigel® | [94] |
| Trophoblast organoids | Placenta in 6–7 weeks gestation from normal pregnancy | Mechanical (chopping) and enzymatic (Trypsin with DNase I) | 60% Matrigel® | [100] |
| Placenta in 6–9 weeks gestation from normal pregnancy | Mechanical (scraping) and enzymatic (Trypsin-EDTA and collagenase V) | 100% Matrigel® | [101] |
The type of tissue, methods used for isolating, and culturing primary cells for the establishment of organoids from normal human FRT tissues are listed.