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. 2020 Oct 7;124(2):484–493. doi: 10.1038/s41416-020-01112-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a RMF-EG cells were transfected with control or KDM2A shRNA. After 48 h, stable cell lines were selected by antibiotics. The KDM2A protein level in the control or KDM2A-depleted cell lines was compared by western blotting. b MDA-MB-231 breast cancer cells were co-injected without or with control or KDM2A-depleted RMF-EG mammary fibroblasts into the fourth mammary fat pads of 6-week-old female BALB/cAnN.Cg-Foxn1nu/CrlNarl mice. The tumours were harvested three weeks after injection. c Tumour weights were measured and were expressed as Mean ± SD. d The expression of β-galactosidase (GLB1) in tumour fibroblasts was investigated by immunohistochemical staining and the number of GLB1-positive fibroblasts in a 400× histological field was expressed as Mean ± SD. e The expression of PD-L1 in CAFs was studied by immunohistochemical staining. The number of PD-L1-positive CAFs was counted in a 400× histological field and was expressed as the percentage of the total CAFs counted. *P < 0.05; **P < 0.01; ***P < 0.001.