Fig. 2. Upregulation of proprotein convertase Pcsk5 mRNA in the LHb with CSDS.

a Coronal section of the murine epithalamus with hematoxylin and eosin staining showing areas processed for RNA-seq analysis (dashed lines). Scale bar, 100 μm. b Venn diagrams showing the intersection of differentially expressed genes in the LHb of defeated mice with susceptibility (Susceptible) versus nondefeated (blue) and Resilient groups (green). c Table showing the genes with fold-change in expression of the susceptible group over the nondefeated group and resilient groups. Results of the statistical analyses with p-values and false discovery rate (FDR) for three group comparison in likelihood ratio test and with p-values and correlation coefficient (R) in Pearson’s correlation analysis are presented. Genes were chosen according to the fold-change between groups > 1.3, p < 0.05, logCPM > 3, and FDR < 0.25. d Schematic of the signaling pathways for activation of MMP2 (gelatinase A) and MMP9 (gelatinase B) according to the information available on reactome pathway database “Activation of Matrix Metalloprotease”. e Table showing fold-changes and p-values of expression of genes known to activate MMP2 (left) and MMP9 (right) in susceptible and resilient groups over the nondefeated group. ND not detected. NA not available.