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. 2020 Aug 18;28(1):349–366. doi: 10.1038/s41418-020-00604-y

Fig. 6. Caspase-2-S384E expressing cells fail to cleave MDM2 and show increased polyploidy following cytokinesis block with blebbistatin.

Fig. 6

GFP-expressing U2OS (sWT) and U2OS-CASP2−/− expressing GFP (sKO), GFP-caspase-2-C320G (sC320G) or GFP-caspase-2-S384E (sS384 #1 and #2), were treated with DMSO or 50 µM blebbistatin (Bleb) for 48 h and subjected to immunoblot and DNA content analysis. a Representative immunoblots (from five independent experiments) of cell lysates from treated stable cell lines. Antibodies used for immunoblotting are as indicated. β-actin was used as loading control. b Graph comparing percentage of cells with polyploid (>4N) DNA content following Bleb treatment. The percentage of polyploid cells (>4N) was calculated using FACS Express 6 cell cycle analysis module. Mean ± SEM; n = 5. *p < 0.05; **p < 0.01 compared to sWT + Bleb.