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. 2020 Jun 17;27(12):3289–3306. doi: 10.1038/s41418-020-0578-0

Fig. 6. vIRF1 promotes CD82 degradation via recruiting E3 ubiquitin ligase AMFR to interact with CD82.

Fig. 6

a qPCR analysis of CD82 mRNA expression in HUVECs transduced with 2 MOI lentiviral vIRF1 (vIRF1) or control lentivirus (pHAGE). b Western blotting analysis of CD82 expression in vIRF1-expressing HUVECs treated with CHX (80 μg/ml) for 0, 4, 8, and 16 h. c Results were quantified in b. d Western blotting analysis of CDCP1 expression in vIRF1-expressing HUVECs treated with MG132 (25 μM) for 8 h. e Results were quantified in d. f Immunoprecipitation assay of CD82 ubiquitination in HUVECs transduced with 2 MOI lentiviral vIRF1 (vIRF1) or control lentivirus (pHAGE). g Western blotting analysis of AMFR and CD82 expression in HUVECs transduced with 2 MOI lentiviral vIRF1 (vIRF1) or control lentivirus (pHAGE). h Immunoprecipitation analyses of the interaction between CD82 and AMFR. i, j Immunoprecipitation analyses of the interaction between CD82 and vIRF1. The light chain band was used as a control to show the equal amounts of antibodies used for immunoprecipitations. k Immunoprecipitation analyses of the interaction between AMFR and vIRF1. The light chain band was used as a control to show the equal amounts of antibodies used for immunoprecipitations. Data were shown as mean ± SD. ***P < 0.001, Student’s t-test.