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. 2020 Jul 31;46(2):394–403. doi: 10.1038/s41386-020-0783-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Representative FSCV color plots for each diet group (non-restricted, NR; protein-restricted, PR) depicting current changes (color) over time (x-axis; in sec) as a function of the recording electrode holding potential (y-axis; −0.4 to +1.3 V and back) in response to single pulse electrical stimulation (0.7 mA, 0.2 ms; vertical white dashed lines). White line insets represent voltammograms for each color plot. b Left: NAc [DA]_o versus time (in nM; mean ± SEM) in slices from adult and adolescent NR and PR rats, aligned to the single pulse electrical stimulation (black box). [DA]_o peaks and AUC were calculated after the end of the stimulation; Right: Mean [DA]_o release (AUC calculated on a 5 sec time window post stimulation) evoked by single pulse stimulation in the NAc. c Cumulative distribution of single pulse evoked NAc [DA]_o AUC in adult (top) and adolescent (bottom) groups. d Mean [DA]_o peak evoked by single pulse stimulation in the NAc. e Average T₈₀ (time for 80% decay from [DA]_o peak) in the NAc. Adults NR (black, n = 8/5 rats), Adults PR (blue, n = 7/3 rats), Adolescents NR (gray, n = 9/5 rats) and Adolescents PR (orange, n = 5/3 rats). Bars show means ± SEM and circles show individual (e.g., recording site) data points. *p < 0.05 Diet effect (Student’s unpaired t test), ^#p < 0.05 Age effect (two-way ANOVA).